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Title: Identification and Elucidation of the CIN2 gene (Advisor: Dr. Joseph J. Kieber - Mentor: Dr. Hyun Sook Chae)
Dana Thi Tang Plant development is controlled by the interactions that occur among several different hormones; cytokinin, in particular, is one of the hormones found in the flowering plant Arabidopsis thaliana and is involved in the induction of another hormone, ethylene. To better understand this induction and the regulatory mechanism of ethylene biosynthesis, several mutants have been isolated and studied. One mutant, cin (cytokinin insensitive) 2, does not display the triple response when treated with cytokinin and also shows a decrease in ethylene production under a variety of conditions; thus, CIN2 is likely to encode a general regulator of ethylene biosynthesis. The cloning of the CIN2 gene with the use of a map based approach of 740 F2 lines from a backcross of cin2 in the ecotype WS to the distinct ecotype Col will provide insight into the mechanism regulating ethylene biosynthesis and can be used to increase the life and quality of different agricultural plants. |
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