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Title: The Cell Cycle and Cross-Resistance of Etoposide-Resistant Cells to 1-ß-D- (Advisor: Dr. Barry Goz - Sponsor: Dr. Lillie Searles)
Tanya Rogo Drug resistance is major problem in the treatment of cancer. Understanding why cells develop resistance is therefore vital to improving the effectiveness of cancer drugs. SV28 are baby hamster cells that have been transformed by the oncogenic virus SV40. V5ER cells are a drug-resistant cell line derived from SV28 cells by growing them in increasing concentrations of the cancer drug etoposide. Etoposide works by inhibiting the action of topoisomerase II, a protein that allows DNA replication to occur by preventing the separating DNA strands from kinking up during replication. Cells that are resistant to the action of etoposide (such as V5ER cells) have been found to have reduced topoisomerase II. Furthermore, these cells are also cross-resistant to the drug AraC (1-ß-D- arabinofuranosyl cytosine). The hypothesis is that the V5ER cells have increased resistance because they regulate their cell cycle differently as a result of having reduced topoisomerase II. |
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