Title: Characterization of pale1 mutant in Arabidopsis

(Research Advisor: Dr. Joseph Kieber, Mentor: Dr. Aaron Rashotte)

Michael McCasland

I am working on characterizing the pale1 gene mutation in Arabidopsis, a plant model system.  This mutant has numerous abnormalities including smaller leaves with altered shape, delayed flowering, and a pale color.  In addition, pale1 seedlings have difficulty perceiving red light, suggesting a connection between the appearance of pale1 and a deficiency in light perception.  Measurements were made that show pale1 as having reduced levels of chlorophyll, as well as starch granules, a byproduct of photosynthesis.  Additionally, transmission electron microscope pictures were taken showing that pale1 is missing an entire cell layer, explaining the reduction in chlorophyll.  I am in the process of mapping the pale1 mutation in the genome to determine which gene is disrupted.  Further understanding of this mutant and the function of the gene responsible may help to give a better understanding of leaf development and the process of photosynthesis.