Movies
Movie 1
Mitotic PtK1
cell induced to enter anaphase precociously by Mad2DC microinjection. Two lagging chromosomes are present in
anaphase
The tip of the microinjection
needle is visible in the first frame. Phase contrast pictures where acquired
every 30 seconds. Each frame is played for 1/10th of a second. The cell enters
anaphase precociously and displays two lagging chromosomes.
Movie 2
Mitosis in PtK1
cell expressing GFP-g-tubulin (red)
Phase contrast and fluorescence images were acquired almost simultaneously
every 2 minutes, using a 4X4 binning. Each frame is played for 1/10th of a
second. Centrosomes (red) are well separated and localized on opposite sides of
the nucleus when nuclear envelope breaks down.
Movie 3
Mitotic PtK1 cell
expressing GFP-g-tubulin
(red) and showing a lagging chromosome in anaphase
Phase contrast and fluorescence
images were acquired almost simultaneously every 2 minutes, using a 4X4
binning. Each frame is played for 1/10th of a second. This cell shows a
chromosome lagging behind when all the others migrate to the spindle poles in
anaphase.
