Sequential images of a yku70 mutant cell (strain KBY3124) containing a lacO marked dicentric chromosome III showing critical stages in the formation of a stretched lacO array.

Sequential images of a KBY3124 cell showing steps in the resolution of a stretched lacO array. 

Unequal distribution of lacO DNA is observed in KBY3124 cells following the resolution of DNA stretching events

Breakage and condensation of a stretched lacO DNA filament 

 
LacO between two centromeres in anaphase. The lac operators are evenly spaced along the 10kb of the lac operator array. When the arrays are stretched in vivo, alternating regions of bound (fluorescent) and unbound (not fluorescent) chromatin can be visualized. As shown in the time-lapse, bound and unbound domains can be delineated. These fluctuations reflect heterogeneity in lac-repressor binding that become apparent in analysis of single molecules.

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