ASH1 mRNA Localization in 3 Acts

Figure 7: GFP-Yta6 localizes to the maternal cortex
 

	GFP-Yta6 in Wildtype (YEF473a) cells. Video Sequence 9. Spots of GFP-Yta6 remain on the maternal cortex until late in the cell cycle. Anaphase onset can be observed via HistoneH4-CFP staining of the chromatin, and compared to the accumulation of GFP-Yta6 in the bud.  Panels:  Top/Left: brightfield Top/Right: GFP-Yta6 Bottom/Left: Chromatin (HistoneH4-CFP)  Botton/Right: Composite of GFP + CFP
	GFP-Yta6-ASH1 3'UTR with CP-GFP in Wildtype (YEF473a) cells. Video Sequence 10.  The localization of the GFP-YTA6-ASH1 mRNA and protein can be observd during the accumulation of Yta6-GFP-ASH1 fusion protein into the bud.