Mail to Instructor: Sarah Grant
Mail to TA: Vanessa Gonzalez Perez
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August 25/26 No labs - classes and labs start next week.
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Aug. 31. Genes, Alleles and Epistasis
For review: Genes, and alleles, complementation : Hartwell et al., 3rd edition Chapter 2 pp. 13-27, Chapter 5 pp. 127-130, Chapter 7 pp. 225-226.
Sept. 1/2. Lab 1: Mendelian Genetics using Fast Plants
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Please also look at the FastPlants web site: http://www.fastplants.org.
Report due Sept. 15/16.
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Sept. 7. Labor Day - No lecture but labs will be held this week.
Sept. 8/9. Lab 2: Pipetteman and and Titration Exercise, Genes and Alleles in yeast. Students will need to come back briefly between 9 and 5 on day 2, 3 and 4 (the next Monday for Wed class) after the lab period to finish the experiment and collect results.
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Readings from Manny, T., Davis, L., Johnson, B., Manney, M., Montelone, B., Weaver, L., and Williamson, B. 1997. A Classroom Guide to Yeast Experiments Kansas State University. Distributed by Carolina Biologicals.
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Reading - A Closer Look at Adenine-requiring Mutants in Yeast--
Report on Yeast Alleles due Sept. 22/23.
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Sept. 14. Mouse Models and Epistasis.
For Review: Hartwell Chapter 3 pp. 56-71.
Reading for discussion in class: Bowman et al., 1991
Sept. 15/16. Epistasis using mice, Guest speaker: Dr. David Aylor, UNC Dept of Genetics.
Report due Sept. 29/30.
Report on Mendelian Genetics Using Fast Plants due in lab this week.
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Sept. 21. Yeast as a Model System: Guest lecturer Dr. Elaine Yeh.
For Review: Hartwell Chapter 7 pp. 208-224.
Reading - A Closer Look at Biological Consequences of UV exposure
Optional Reading Mouse mutagenesis: Du et al., 2004
Optional Reading Mouse mutagenesis: Beutler et al., 2005
Sept. 22/23. Mutation in Yeast. Students will need to come back between 9 and 5 on day 3 after the lab period to collect results.
Report due Oct. 6/7.
Report on Genes and Alleles in Yeast due in lab this week.
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Sept. 28 Bacterial Genetics
Reading for discussion in class: Tatum and Lederberg 1947.
For Review: Hartwell chapter 15 pp. 543-562.
Optional, for interest:
E. coli genome comparisons Brzuszkiewicz et al 2006.
Pseudomonas syringae genome comparisons Chang et al 2005.
Sept. 29/30 Conjugation Lab. Students will need to come back briefly between 9 and 5 on day 2 or 3 after the lab period to collect results.
Report due Oct. 13/14.
Report on Epistasis in Mice Lab due in lab this week.
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Oct. 5 C. elegans as a model system and epigenetics
For Review: Hartl and Jones pp. 558-562.
Reading - dsRNA blocks gene expression Fire and Mellow 1998
Reading RNAi mechanism Mellow and Conte 2004
Reading - RNAi and chromatin Verdel et al., 2009
Optional Reading - Epigenetics Eymary et al., 2009.
Oct. 6/7 and Oct. 13/14 C. elegans Genetics and RNAi. This lab must extend beyond the set lab days. Students will need to come in for a short time almost every of the first week and several other days up to day 12 after the first lab period as described in the Protocol flow chart below. This will involve coming in on both weekends following the first lab period. The weekend sessions will require about one hour but depending on your class you may need to come in both Saturday and Sunday. Look at the flow chart. You can arrange with others in the class to share the work as long as the experiment moves forward properly. The TA will arrange times to meet for these extra sessions.
Report due: Oct. 27/28.
Report on Mutation in Yeast Lab due in lab this week.
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Oct. 12 University Day - Class cancelled
Oct. 13/14 C. elegans genetics and RNAi lab will continue.
Research Paper Topics due Oct. 14, send by email to Dr. Grant by 5 pm Oct. 14.
Report on C. elegans lab due: Oct 27/28.
Report on Conjugation Lab due in lab this week.
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Oct. 19 Three point cross and mapping in humans
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For review: Drosophila: Hartwell Chapter 20 pp. 732-749, Sex-linked genes: Chapter 4 pp. 105-112, 3-point cross: Chapter 5 pp. 123-141, Cystic fibrosis, Chapter 5 pp. 142-143, map based cloning in humans: Chapter 11 pp. 408-419.
Oct. 20/21, Nov. 3/4 and Nov. 17/18 Drosophila 3-point Cross
Report on Drosophila lab due in lab Dec. 1/2. ____________________________________________________________________
Oct. 26 Midterm covers up to C. elegans as a Model System and Epigentics lecture and C. elegans lab.
Oct. 27/28 DNA Cloning Lab. Students will need to come back briefly between 9 and 5 on day 2 and day 3 after the lab period to finish the experiment and collect results.
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For review: Hartwell Chapter 9 pp. 303-326.
Report due: Nov 10/11.
Report on C. elegans lab due in lab this week.
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Nov. 2 Drosophila as a Model System Guest lecturer: Dr. Bob Duronio
Nov. 3/4 Drosophila lab continues, test cross, score for sex-linked traits.
1-2 page outlines for research papers due November 6, email to Dr. Grant by 5 pm.
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Nov. 9 DNA markers and Forensics
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For review: Hartwell Chapter 9 pp.327-330.
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Reading for discussion in class: Lander and Ellis News and Views 1998.
Nov. 10/11 DNA Markers and Forensics Lab. Students will need to come back briefly between 9 and 5 on day 2 and day 3 after the lab exercise to finish the experiment and collect results.
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Reading for lab: Nakamura et al 1988.
Results will be discussed in class Nov. 16. No lab report.
Report on Cloning Lab due in lab this week.
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Nov. 16 Map Based Cloning
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Optional reading:
Nov 17/18 Score 3-point Cross
Report on Drosophila lab due in lab Dec 1/2.
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Nov. 23 Association Mapping for Human Diseases Guest lecturer: Dr. Karen Mohlke
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for review: Hartwell Chapter 11 pp. 423-425.
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Reading: Review
There will be no lab class this week because of Thanksgiving.
Research paper deadline extended to Dec. 4
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Nov. 30 Map Based Cloning Continues
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for review: Hartwell Chapter 10 pp.354-365.
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Reading - Lukowitz et al., 2000
Dec. 1/2 Map-Based Cloning Lab.
Report on Drosophila Lab due in lab this week.
Research papers due Dec. 4 at 3:30 pm. Bring to lab this week or put in Dr. Grant's mailbox in Coker Hall room 208A.
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Dec. 7 Results of Map Based Cloning, general review of course material
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Dec. 14 Final Exam in 128 Wilson Hall 12:00 noon - 3:00 pm. Covers entire course.
