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Current Topics in Cell
Division
Text
book: Giant
molecules: here, there, and everywhere…. A.
Y.
Grosberg and A.R.
Khohlov Syllabus for Bio 642: Current Topics in Cell Biology: Mechanical Processes in Cells Mechanical forces
are generated
in almost every cellular process, from replication to transcription to
translation to segregation. Mechanical force can alter the fate of an
enzymatic
reaction and a given process in profound ways. Cell biologists are just
starting
to measure forces in vivo and to
study how force shapes cellular events. This course will focus on the
segregation apparatus as a biochemical machine that uses force to
segregate
chromosomes. We will explore the mechanical properties of the major
classes of
molecules in the mitotic spindle, including microtubules,
microtubule-based
motor proteins, DNA, histones and chromosome architectural proteins.
The course
will culminate in developing computer models of mitotic spindle
function in
yeast, frogs and mammals. There's
Plenty of Room at the Bottom - Talk by Richard Feynman Course
Readings/Lectures:
Jan. 15: Boone C, Bussey H, Andrews BJ. Exploring genetic interactions and networks with yeast. Nat Rev Genet. 2007 Jun;8(6):437-49. Review. Jan. 17 (Lecture): Purcell E.M. Life at low Reynolds Number. American Journal of Physics 1977 Jan. Vol 45 No.1 Jan. 29 (Problem Set 1 - Due Feb 5): Bustamante C, Bryant Z, Smith SB. Ten years of tension: single-molecule DNA mechanics. Nature. 2003 Jan 23;421(6921):423-7. Review. February 5: Answers to Problem Set 1 February 7: Theriot, Julie A. The Polymerization Motor. Jun, S., and Mulder, B. Entropy-driven spatial organization of highly confined polymers: Lessons for the bacterial chromosome. February 12: Marko and Siggia Polymer Models of Meiotic and Mitotic Chromosomes Poirier and Marko Micromechanical studies of mitotic chromosomes |
| February 19:
Lecture (Powerpoint) April 25: Gardner et al. Kinesin-5 motors mediate chromosome congression by promoting disassembly of longer microtubules Figures, Bustamante Powerpoint The Path of DNA in the Kinetochore Powerpoint Gardner et al. Kinesin-5 Motors Promote Length-Dependent Microtubule Disassembly During Mitosis Powerpoint How can dynamic kinetochore movements result in stable kinetochore cluster positioning in metaphase? Powerpoint Chromosome Mechanics Powerpoint Entropic Spring Powerpoint Reynolds Number Powerpoint |
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